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	Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy
	Zhao, Xiang1; Zhang, Mingkun2; Wei, Dongshan2; Wang, Yunxia1; Yan, Shihan2; Liu, Mengwan2; Yang, Xiang1; Yang, Ke1; Cui, Hong-Liang2; Fu, Weiling1
	2017-10-01
摘要	The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/mu L, which is approximately equal to the optimal immobilized concentration of aptasensors. (C) 2017 Optical Society of America
DOI	10.1364/BOE.8.004427
发表期刊	BIOMEDICAL OPTICS EXPRESS
ISSN	2156-7085
卷号	8期号:10页码:4427-4437
通讯作者	Cui, HL (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China.
收录类别	SCI
WOS记录号	WOS:000412052000010
语种	英语