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Hierarchical Au nanoarrays functionalized 2D Ti2CTx MXene membranes for the detection of exosomes isolated from human lung carcinoma cells
You, Qiannan1,2; Zhuang, Linlin1,2; Chang, Zhimin2; Ge, Mingfeng2; Mei, Qian2; Yang, Li3; Dong, Wen Fei1,2
2022-11-15
摘要Exosome is considered an important biomarker of liquid biopsy in early cancer screening, which can reflect the physiological and pathological status of cancer cells. Herein, we construct a novel electrochemical biosensor based on hierarchical Au nanoarray-modified 2D Ti2CTx MXene membranes for sensitive detection of exosomes. Ti2CTx MXene nanosheets were fabricated as the building blocks for preparing 2D membranes as the sensing platform via vacuum filtration. To enhance the conductivity of the MXene membrane, for the first time, hierarchical Au nanoarrays were further deposited in situ on the MXene membrane surface. The combination of MXene membrane with a large specific area and hierarchical Au nanoarrays with excellent conductivity make higher electrocatalytic and more active sites in aptamer immobilization. In this strategy, the composite mem-brane modified by EpCAM recognized aptamer can specifically capture target exosomes, meanwhile, these target exosomes anchor aptamer for CD63 to further enhance the sensing sensitivity and accuracy of the biosensor. As a result, the biosensor achieved high sensitivity and reliable performance for exosome sensing, with a low detection limit (58 particles/mu L) in the linear range of 1 x 10(2) to 1 x 10(7) particles/mu L. In addition, this biosensor showed satisfactory electrochemical stability and anti-interference ability for the detection of exosomes in real serum samples.
关键词Hierarchical nanoarrays Au-MXene membrane Dual amplification Exosomes detection Electrochemical biosensor
DOI10.1016/j.bios.2022.114647
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号216页码:8
通讯作者Yang, Li(ylyhp@126.com) ; Dong, Wen Fei(wenfeidong@sibet.ac.cn)
收录类别SCI
WOS记录号WOS:000854894900004
语种英语