中文版 | English
CSpace
Evaluation of simultaneous autotrophic and heterotrophic denitrification processes and bacterial community structure analysis
Xu, Guihua1,2; Peng, Jingjing1; Feng, Cuijie1; Fang, Fang1; Chen, Shaohua1; Xu, Yuanjian2; Wang, Xingzu2
2015-08-01
摘要This study demonstrated that a combined heterotrophic and autotrophic denitrification (HAD) process is highly effective for the simultaneous removal of acetate, nitrate, and sulfide at an efficiency of 100, 80, and 100 %, respectively. In the HAD system, simultaneous sulfide, acetate, and nitrate removals were observed, which indicated that heterotrophic and autotrophic denitrification occurred simultaneously. When the sulfide was existed in HAD reactor, the main product of sulfide biooxidation was S-0. Once the sulfide was exhausted, the sulfate concentration in the HAD reactor increased and became the main end product. These results provided an alternative method to control the end sulfide biooxidation product by online monitoring sulfide concentration. Nearly half (43 %) of the total clones in our mix-trophic reactor were amphitrophy denitrifiers. The autotrophic denitrifiers, heterotrophic denitrifiers, and amphitrophy denitrifiers coexisted in the HAD reactor to complete the denitrification process. Retrieved bacterial 16S rRNA gene clones affiliated with uncultured Xanthomonadaceae, Thauera, Thiobacillus, and Chromatiales were dominant.
关键词Sulfide biooxidation Autotrophic denitrification Heterotrophic denitrification Microbial community structure 16S rRNA gene
DOI10.1007/s00253-015-6532-2
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号99期号:15页码:6527-6536
收录类别SCI
WOS记录号WOS:000357649200030
语种英语