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	Occurrence and prevalence of antibiotic resistance in landfill leachate
	Wang, Yangqing; Tang, Wei; Qiao, Jing; Song, Liyan
	2015-08-01
摘要	Antibiotic resistance (AR) is extensively present in various environments, posing emerging threat to public and environmental health. Landfill receives unused and unwanted antibiotics through household waste and AR within waste (e.g., activated sludge and illegal clinical waste) and is supposed to serve as an important AR reservoir. In this study, we used culture-dependent methods and quantitative molecular techniques to detect and quantify antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in 12 landfill leachate samples from six geographic different landfills, China. Five tested ARGs (tetO, tetW, bla(TEM), sulI, and sulII) and seven kinds of antibiotic-resistant heterotrophic ARB were extensively detected in all samples, demonstrating their occurrence in landfill. The detected high ratio (10(-2) to 10(-5)) of ARGs to 16S ribosomal RNA (rRNA) gene copies implied that ARGs are prevalent in landfill. Correlation analysis showed that ARGs (tetO, tetW, sulI, and sulII) significantly correlated to ambient bacterial 16S rRNA gene copies, suggesting that the abundance of bacteria in landfill leachate may play an important role in the horizontal spread of ARGs.
关键词	Landfill Landfill leachate Antibiotic resistance (AR) Antibiotic-resistant bacteria (ARB) Antibiotic-resistant genes (ARGs)
DOI	10.1007/s11356-015-4514-7
发表期刊	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN	0944-1344
卷号	22期号:16页码:12525-12533
收录类别	SCI
WOS记录号	WOS:000358579700052
语种	英语