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Graphene-based field-effect transistor biosensor for prostate-specific antigen detection
Kong, Xiangdong1,2; Wang, Yunjiao2,3; Huang, Deping2,3; Li, Xin2,3; Shi, Biao2,3; Zhou, Daming2,3; Tian, Rong2,3; Tlili, Chaker2,3; Wang, Deqiang2,3
2024-10-01
摘要The development of a graphene-based field-effect transistor (GFET) aptasensor for prostate-specific antigen (PSA) detection. We present the novel use of 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) to functionalize the graphene surface, enabling covalent bonding of amine-functionalized PSA-binding aptamers. Under optimized conditions, the shift of the GFET aptasensor's Dirac point voltage exhibited a systematic variation with PSA concentrations. The sensor demonstrated a relatively wide linear dynamic range spanning from 100 fM to 100 nM, with a detection limit of 3.5 pM. Importantly, it displayed satisfactory specificity against potential interfering proteins and demonstrated good reproducibility and stability. Furthermore, we successfully applied the proposed method for the quantification of PSA in human serum samples, indicating its potential for bioanalysis and clinical diagnostics. The GFET aptasensor's potential in medical diagnostics and personalized healthcare, positioning it as a promising tool for rapid and accurate biomarker detection.
关键词Field-effect transistor Prostate-specific antigen Graphene Aptamer PBASE
DOI10.1016/j.microc.2024.111346
发表期刊MICROCHEMICAL JOURNAL
ISSN0026-265X
卷号205页码:8
通讯作者Tian, Rong(tianrong@cigit.ac.cn) ; Tlili, Chaker(chakertlili@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:001290211200001
语种英语