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Physiological roles of mycothiol in detoxification and tolerance to multiple poisonous chemicals in Corynebacterium glutamicum
Liu, Ying-Bao1,2; Long, Ming-Xiu3; Yin, Ya-Jie4; Si, Mei-Ru1,2; Zhang, Lei1,2; Lu, Zhi-Qiang5; Wang, Yao1,2; Shen, Xi-Hui1,2
2013-06-01
摘要Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most eukaryotes. In this research, we explored the impact of intracellular MSH on cellular physiology by using MSH-deficient mutants in the model organism Corynebacterium glutamicum. We found that intracellular MSH contributes significantly to resistance to alkylating agents, glyphosate, ethanol, antibiotics, heavy metals and aromatic compounds. In addition, intracellular MSH is beneficial for withstanding oxidative stress induced by various oxidants in C. glutamicum. This study greatly expanded our current knowledge on the physiological functions of mycothiol in C. glutamicum and could be applied to improve the robustness of this scientifically and commercially important species in the future.
关键词Corynebacterium glutamicum Mycothiol Oxidative stress Detoxification
DOI10.1007/s00203-013-0889-3
发表期刊ARCHIVES OF MICROBIOLOGY
ISSN0302-8933
卷号195期号:6页码:419-429
通讯作者Shen, XH (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China.
收录类别SCI
WOS记录号WOS:000319762200006
语种英语