KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Reproductive effects linked to DNA methylation in male zebrafish chronically exposed to environmentally relevant concentrations of di-(2-ethylhexyl) phthalate | |
Ma, Yan-Bo1,2 ; Jia, Pan-Pan1,2; Junaid, Muhammad1,2; Yang, Li1; Lu, Chun-Jiao1; Pei, De-Sheng1,2
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| 2018-06-01 | |
| 摘要 | Di-(2-ethylhexyl) phthalate (DEHP) possesses the potential to interfere with the male reproductive endocrine system in mammals; however, its reproductive toxicity in male zebrafish and associated epigenetic studies have not been explored. In this study, three-month-old male zebrafish were exposed to environmentally relevant concentrations of DEHP (0, 10, 33 and 100 mu g/L for 3 months, and then the impact on the reproduction of males and the underlying mechanism were investigated. Histological testing showed that an exposure concentration of 100 mu g/L DEHP significantly inhibited spermatogenesis, with an associated decline in capability to fertilize untreated oocytes. Electron microscopic examinations also revealed noticeable damage to the testicular ultrastructure at the 100 mu g/L DEHP exposure level. In addition, exposure to 33 and 100 mu g/L of DEHP resulted in a decline of circulating testosterone (T) and an increase in the level of 17 beta-estradiol (E2), both of which were possibly derived from the downregulation of cypl7a1 and hsd1763 genes and the upregulation of the cyp19a1a gene in the gonads. The DNA methylation statuses of these genes were altered within their promoter regions. A significant increase in global DNA methylation in both the male testes and their offspring larvae was observed at higher exposure concentration of DEHP. Our findings demonstrate that exposure to environmentally relevant concentrations of DEHP can damage the testes, disturbe the sex hormones production, and inhibite spermatogenesis, which ultimately impairs the reproduction of male zebrafish. (C) 2017 Elsevier Ltd. All rights reserved. |
| 关键词 | Di-(2-ethylhexyl) phthalate DNA methylation Male zebrafish Reproduction toxicity |
| DOI | 10.1016/j.envpol.2017.11.025 |
| 发表期刊 | ENVIRONMENTAL POLLUTION
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| ISSN | 0269-7491 |
| 卷号 | 237页码:1050-1061 |
| 收录类别 | SCI |
| WOS记录号 | WOS:000431158900107 |
| 语种 | 英语 |
