中国科学院重庆绿色智能技术研究院机构知识库

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	Ultra-sensitive fluorescent and colorimetric detection of UO22+based on dual enzyme-free amplification strategies
	Yun, Wen1; Wu, Hong1; Liu, Xingyan1; Zhong, Haixia1; Fu, Min1; Yang, Lizhu2; Huang, Yu3
	2018
摘要	A novel fluorescent and colorimetric strategy with enzyme-free dual amplification was developed for ultra-sensitively detection of uranyl in river waters samples. The enzyme-strand recycling and hairpin catalytic assembly (HCA) reaction were used as dual enzyme-free amplification strategies. Three hairpins labeled with fluorophore can absorb on the surface of gold nanoparticles (AuNPs) to prevent them from aggregation in salt solution and quench the fluorescent signal simultaneously. In the presence of UO22+, the HCA reaction between hairpins was induced to form rigid DNA triangles, resulting in the aggregation of AuNPs with a red to blue color change and a “turn-on” fluorescent signal. The limit of detection was as low as 0.1 pM. Importantly, this strategy provided colorimetric and fluorescent dual signals for lower false-positives and higher anti-interference ability. It was successfully used for detection of uranyl in real waters samples. © 2017 Elsevier B.V.
DOI	10.1016/j.snb.2017.08.205
发表期刊	Sensors and Actuators, B: Chemical
ISSN	09254005
卷号	255页码:1920-1926
收录类别	EI
语种	英语